Journal: bioRxiv
Article Title: CRISPR-mediated correction of skeletal muscle Ca 2+ handling in a novel DMD patient-derived pluripotent stem cell model
doi: 10.1101/2022.02.17.480850
Figure Lengend Snippet: (A) Time-course expression levels of genes within HALLMARK_MYOGENESIS that regulate muscle contraction, including TNNC2 , TNNI1 , TNNI2 , TNNT1 , TNNT3 and TPM2 . Wald test with Benjamini-Hochberg method for multiple comparison adjustment (* padj < 0.05, ** padj < 0.01, *** padj < 0.001). TPM, Transcripts Per Million. Each condition has 4 biological replicates. (B-D) Analysis of myotube contraction velocity using Particle Image Velocimetry. In response to acetylcholine (Ach) stimulation, DMD-K2957fs myotubes had significantly slower velocity magnitude than BIONi010-C (B) or CORR-K2957fs (C) myotubes, whereas no significant difference of velocity between BIONi010-C and CORR-K2957fs myotubes (D). Data represent mean ± SEM (n= 9; at least three independent experiments). Two-way ANOVA, Sidak test (** p < 0.01; *** p < 0.001).
Article Snippet: To do this, we measured the velocity of myotube contraction in response to acetylcholine stimulation using particle image velocimetry (PIV) tool in MATLAB, PIVlab [ ].
Techniques: Expressing